abstract：:Eight polymorphic restriction enzyme sites at phenylalanine hydroxylase locus from the parental chromosomes in Polish families with phenylketonuria were analyzed. Among 28 chromosomes studied, we identified haplotypes found within the Danish population. Haplotype 2 has been found in 25% of affected alleles. One of the...

journal_title：Acta biochimica Polonica

authors： Jaruzelska J,Borski K,Riess O,Blin N,Słomski R

更新日期：1989-01-01 00:00:00

abstract：:Unusual chemical properties of hypermodified nucleosides N6-(threoninocarbonyl)adenosine (t6 A) located at position 37 and 3-(3-amino-3-carboxypropyl)uridine (acp3U) located at position 20:1 have been utilized for the introduction of photoreactive azidonitrophenyl probes to the anticodon loop and to the dihydrouridine...

journal_title：Acta biochimica Polonica

authors： Podkowinski J,Dymarek-Babs T,Gornicki P

更新日期：1989-01-01 00:00:00

abstract：:The rate of elimination from rat and goat circulation of 125I-labelled goat haemoglobin and its complexes with human and goat haptoglobin in goat circulation, was studied. The half-life time t1/2 of haemoglobin in rat circulation was 1.5h, whereas in goat circulation -- 4 h, and was 10 times shorter than t1/2 for its ...

journal_title：Acta biochimica Polonica

authors： Osada J,Nowacki W

更新日期：1989-01-01 00:00:00

abstract：:Bovine vitreous humour contains 0.6 +/- 0.04 mg of proteins per cm3. Three main protein fractions were obtained by CM-cellulose chromatography and their homogeneity was confirmed by isoelectric focusing and SDS-gel electrophoresis. The Mr and pl values of the separated fractions were: fraction I, 67,000 and 4.02; frac...

journal_title：Acta biochimica Polonica

authors： Wolańska M,Bańkowski E

更新日期：1989-01-01 00:00:00

abstract：:To establish the identity of testis-specific hsp70 gene-related transcript, the expression pattern of the hsp70 gene family in testis and liver of rats subjected to whole body hyperthermia was investigated. In control liver two hsp70 gene-related transcripts of about 2.2 and 2.5 kb were detected. Increased body temper...

journal_title：Acta biochimica Polonica

authors： Krawczyk Z,Wisniewski J,Biesiada E

更新日期：1988-01-01 00:00:00

abstract：:Tautomerism of the cytosine molecule is discussed in connection with recent experimental matrix-isolation infrared spectroscopic measurements and recent ab initio calculations of relative stabilities of tautomers and of IR spectra for different tautomeric forms of the compound. Experimental IR spectra in the N-H and O...

journal_title：Acta biochimica Polonica

authors： Kwiatkowski JS,Person WB,Szczepaniak K,Szczesniak M

更新日期：1987-01-01 00:00:00

abstract：:It was shown that some base analogs, like n2Pur and n2oh6Ade (but not n2om6Ade or oh4Cyd) which strongly inhibit growth of dam- cells, mutagenize and preselect dam- populations. As a result dam- mut(-)--devoid of mismatch repair, dam+ revertants, or dam(-)--insensitive to a base analog are exclusively obtained after m...

journal_title：Acta biochimica Polonica

authors： Janion C,Bebenek K,Plewako S

更新日期：1987-01-01 00:00:00

abstract：:Secondary structure of alpha (light) and beta (heavy) chains of human haptoglobin was proposed. The Chou-Fasman predictive method was used in conjunction with the hydrophobic character and hydropathy scale value assigned to each amino acid residue in the primary sequence of the protein. Moreover, by means of hydrophil...

journal_title：Acta biochimica Polonica

authors： Dobryszycka W,Kubisa W,Kubicz Z

更新日期：1987-01-01 00:00:00

abstract：:Liposomes made of sphingomyelin were found to inhibit both ATP-activated and non-activated AMP deaminase from pig brain, in contrast to liposomes made of egg yolk phosphatidylcholine which exhibited an activating effect on the ATP-activated enzyme, being without effect on AMP deaminase in the absence of ATP. Dioleoylp...

journal_title：Acta biochimica Polonica

authors： Woźniak M,Purzycka-Preis J,Kossowska E,Zydowo MM

更新日期：1987-01-01 00:00:00

abstract：:Rat duodenum and jejunum were found to contain three forms of alkaline phosphatase, F1, F2 and F3, and ileum two forms of this enzyme, F1 and F2. The procedure for separation of phosphatase F1, F2 and F3 from jejunum is presented. ...

journal_title：Acta biochimica Polonica

authors： Dupuis Y,Landiharintsoa L,Fournier P,Zelazowska E,Staroń I,Porembska Z

更新日期：1986-01-01 00:00:00

abstract：:A procedure for purification of oestrone sulphate sulphohydrolase from human placenta microsomes was elaborated. The use of Concanavalin-A-Sepharose chromatography made it possible to separate, for the first time, oestrone sulphate sulphohydrolase (Mr 36,000, optimum pH 7.0, Km 5.5 X 10(-5) M, specific activity 1563 n...

journal_title：Acta biochimica Polonica

authors： Gniot-Szulzycka J,Januszewska B

更新日期：1986-01-01 00:00:00

abstract：:The content and type of dolichols in chicken embryo fibroblasts was estimated and the presence of enzymic activities of various dolichyl phosphate sugar transferases was documented. Chicken embryo fibroblasts effectively take up various polyprenoids from the culture medium and catalyse both formation of polyprenyl fat...

journal_title：Acta biochimica Polonica

authors： Krajewska-Rychlik I

更新日期：1985-01-01 00:00:00

abstract：:Methionine synthase reaction may be coupled with thymidylate synthase-catalysed tritium release from [5-3H]dUMP via non-enzymatic reaction of formaldehyde with tetrahydrofolate. A convenient and sensitive assay of methionine synthase activity, based on this principle, is described. ...

journal_title：Acta biochimica Polonica

authors： Rode W,Balińska M

更新日期：1985-01-01 00:00:00

abstract：:The activity of polyhydric alcohol dehydrogenases in Mycobacterium sp. 279 was studied under limitation of zinc in the growth medium. It was found that the activity of ribitol and sorbitol dehydrogenases were markedly lowered and that of D-arabinitol dehydrogenase remained unchanged in the Zn2+-deficient cells. Other ...

journal_title：Acta biochimica Polonica

authors： Szumiło T,Byra A

更新日期：1984-01-01 00:00:00

abstract：:The silk protein synthesis in silk glands of Galleria mellonella is preceded by the increase of total RNA content. The levels of the main RNA classes: 28S and 18S rRNA, tRNA as well as poly(A) + RNA change proportionally to the total RNA pool of glands. The fibroin-like silk protein of molecular weight of about 240 00...

journal_title：Acta biochimica Polonica

authors： Michalik J,Sehnal F,Lassota Z

更新日期：1984-01-01 00:00:00

abstract：:Recombination of subunits of rat liver arginase A1 and rat kidney arginase A4 yielded a product which in polyacrylamide gel electrophoresis and DEAE-cellulose chromatography separated into five proteins with arginase activity. Proteins I and V corresponded in polyacrylamide gel-electrophoresis, DEAE-cellulose chromato...

journal_title：Acta biochimica Polonica

authors： Skrzypek-Osiecka I,Porembska Z

更新日期：1983-01-01 00:00:00

abstract：:Ledakrin, 1-nitro-9-[3'-(N,N'-dimethyl)aminopropyl]aminoacridine administered intraperitoneally at a dose up to 20 mg/kg into sham operated and partially hepatectomized rats inhibited RNA polymerase B activity in the isolated liver nuclei. The average chain length of the in vitro transcript was reduced while the numbe...

journal_title：Acta biochimica Polonica

authors： Krawczyk Z

更新日期：1982-01-01 00:00:00

abstract：:Selectivity of amidination using ornithine as model amino acid was investigated in detail. The results obtained were taken advantage of for studying the reactions with other polyfunctional amino acids: beta-tyrosine, isoserine, 2,3-diaminopropionic acid, 2,6-diamino-7-hydroxyazelaic acid and with the pentapeptide amid...

journal_title：Acta biochimica Polonica

authors： Wojciechowska H,Zgoda W,Borowski E

更新日期：1982-01-01 00:00:00

abstract：:The pools of Arg, Gly, His, Ile, Ser, Glu, Leu, Val and Asp were lower during photosynthesis at 1% O2 concentration. At the same time specific radioactivity of a number of amino acids--following 14CO2 incorporation--was different than that at atmospheric O2 concentration. This is probably due to the inhibition of the ...

journal_title：Acta biochimica Polonica

authors： Niziołek S,Bielawski W,Nalborczyk E

更新日期：1982-01-01 00:00:00

abstract：:1. The amounts of deoxynucleotides incorporated during an extensive replication by DNA polymerase alpha into poly(dA)-oligo(dT)12-18 and damaged DNA containing 2.5 incisions per molecule and 3.7% single-stranded DNA, corresponded to the amounts of non-complexed poly(dA) and single-stranded fraction of DNA, respectivel...

journal_title：Acta biochimica Polonica

authors： Siedlecki JA,Nowak R,Soøtyk A,Zmudzka B

更新日期：1981-01-01 00:00:00

abstract：:Secondary structures of the N-terminal fragments of 18 preproteins were analysed taking advantage of the method of Chou & Fasman (Biochemistry, 1974, 13, 222-245; J. Mol. Biol., 1977, 115, 135-175) and the known sequences of amino acid residues. Positions of alpha-helices, beta-sheet structures and beta-turns in the l...

journal_title：Acta biochimica Polonica

authors： Tłomak P,Nowak K

更新日期：1981-01-01 00:00:00

abstract：:Transcriptionally active and inactive chromatin fractions were isolated from Physarum polycephalum after depolymerization of chromatin with DNAase II or micrococcal nuclease, followed by fractionation in 5 mM-MgCl2. The active fraction of chromatin comprised up to 21% of nuclear DNA and was enriched 22-fold in the lab...

journal_title：Acta biochimica Polonica

authors： Czupryn M,Toczko K

更新日期：1980-01-01 00:00:00

abstract：:Adenylate deaminase (EC 3.5.4.6) has been isolated from the hearts of healthy rats and from the rats with cardionecrosis induced by administration of vitamin D in high doses. Crude extract from the necrotic hearts catalysed adenylate deamination with a higher maximum velocity and a higher apparent Km value than the ex...

journal_title：Acta biochimica Polonica

authors： Zydowo M,Walentynowicz O,Wrzołkowa T

更新日期：1980-01-01 00:00:00

abstract：:Chromatin of lower eukaryote Physarum polycephalum, while showing typical nucleosomal organization, reveals upon digestion with micrococcal nuclease certain features not found in chromatins of higher eukaryotes, the most pronounced of which is the unusual pattern of degradation of core-size DNA, without accumulation o...

journal_title：Acta biochimica Polonica

authors： Staroń K,Jerzmanowski A,Fronk J,Toczko K

更新日期：1980-01-01 00:00:00

abstract：:An analysis has been made, with the aid of 1H NMR spectroscopy, of the solution conformation of the known antimetabolite, 9-beta-D-xylofuranosyladenine (xyloA), and of its 8-bromo analogue. For xyloA, the results point to a strong preference for the sugar ring of the conformation type N (C(3') endo), a relatively low ...

journal_title：Acta biochimica Polonica

authors： Ekiel I,Shugar D

更新日期：1979-01-01 00:00:00

abstract：:Chemical modifications were used to search for catalytically important residues of rat liver arginase. The results of carbamoylation, nitration and diazotization suggest that lysyl and tyrosyl residues are not involved in the catalytic function of arginase. The modification of 5--6 tryptophanyl residues by N-bromosucc...

journal_title：Acta biochimica Polonica

authors： Ber E,Muszyńska G

更新日期：1979-01-01 00:00:00

abstract：:Two different molecular forms of bacteriolytic endo-N-acetylmuramidase (EC 3.2.1.17) were found in the Acanthamoeba castellanii culture. The enzymes were separated by column chromatography on DEAE-Sephadex or by polyacrylamide-gel electrophoresis. In young cultures only one molecular form of the enzyme was selectively...

journal_title：Acta biochimica Polonica

authors： Drozański W

更新日期：1978-01-01 00:00:00

abstract：:1. The lupin cotyledons contain two cytoplasm-specific lysine tRNAs, one chloroplast-specific, one mitochondria-specific species, and three species common to all the cell compartments investigated; the lysine tRNAs were separated by reversed-phase chromatography (RPC-5). 2. The two cytoplasmic lysine tRNAs are predomi...

journal_title：Acta biochimica Polonica

authors： Augustyniak H,Pawełkiewicz J

更新日期：1978-01-01 00:00:00

abstract：:1. The interaction of copper(II) with inosine, 2'-deoxyinosine, 1-methylinosine, 7-deazainosine, 6-methoxypurine riboside and IMP was examined. 2. Copper binds with the purine base of the nucleosides, and in IMP also with the phosphate group. Non interaction with ribose hydroxyl groups was observed. 3. In non-aqueous ...

journal_title：Acta biochimica Polonica

authors： Maskos K

更新日期：1978-01-01 00:00:00

abstract：:Interaction of copper(II) with guanosine, 2'-deoxyguanosine, 1-methylguanosine, 7-methylguanosine and GMP was studied withe use of spectroscopic and magneto-chemical methods. The main site of copper(II) binding in guanosine is nitrogen N-7; participation of N-1 is not excluded. The involvement of carbonyl oxygen in co...

journal_title：Acta biochimica Polonica

authors： Maskos K

更新日期：1978-01-01 00:00:00

abstract：:Human haptoglobin (Hp) of the 1-1 type incorporated one spin or fluorescence marker per molecule; the markers were found in the beta chain. Formation of the complex between spin-labelled Hp and haemoglobin or antibody caused conformational changes in the Hp molecular, evidenced by increased participation in the electr...

journal_title：Acta biochimica Polonica

authors： Osada J,Sawaryn A,Dobryszycka W

更新日期：1978-01-01 00:00:00

abstract：:1. Gluconeogenesis from glutamine, fumarate, pyruvate, glutamine plus fumarate, and glutamine plus pyruvate, was generally higher at pH 7.1 than at pH 7.4 and 7.7, whereas ammoniagenesis did not depend on the pH of the medium. 2. The intermediates of the Krebs cycle decreased ammonia formation from glutamine, raising ...

journal_title：Acta biochimica Polonica

authors： Strzelecki T,Rogulski J

更新日期：1977-01-01 00:00:00

abstract：:alpha-Glycerophosphate dehydrogenase (EC 1.1.99.5) in mitochondria from liver of the triiodothyronine-treated rats is competitively inhibited by phosphoenolpyruvate, glyceraldehyde 3-phosphate and 3-phosphoglycerate, the apparent Ki values for phosphoenolpyruvate being 0.76 mM at pH 7.0, 1.7 mM at pH 7.4 and 3.5 mM at...

journal_title：Acta biochimica Polonica

authors： Swierczyński J,Scisłowski P,Aleksandrowicz Z

更新日期：1977-01-01 00:00:00

abstract：:1. The amino acid composition of wheat germ EF2 differs to some extent from that of elongation factors from mammals and bacteria. 2. The purified wheat germ EF2, similarly as the factors from other sources, is active in the: EF1-dependent polymerization of phenylalanine; ribosome-dependent GTP hydrolysis; binding of g...

journal_title：Acta biochimica Polonica

authors： Twardowski T,Legocki A

更新日期：1977-01-01 00:00:00

abstract：:Some properties of the sulphate transport system and the isolation of sulphate permease mutants in E. coli K12 are described. The gene coding for sulphate permease is located in the same region as the cysA gene in Salmonella typhimurium. ...

journal_title：Acta biochimica Polonica

authors： Karbonowska H,Wiater A,Hulanicka D

更新日期：1977-01-01 00:00:00

abstract：:1. Susceptibility to inhibitors of neutral protease from calf thymus chromatin has been compared with that of trypsin. The chromatin protease reacts stoichiometrically with the inhibitors specific for trypsin (diisopropylfluorophosphate, tosyl-lysyl chloromethane, soybean trypsin inhibitor and Kunitz basic inhibitor f...

journal_title：Acta biochimica Polonica

authors： Kowalska-Loth B,Zieleński J,Toczko K,Chmielewska I

更新日期：1976-01-01 00:00:00

abstract：:In the post-mitochondrial fraction of murine LBN/b leukemic cells, four fractions with DNA polymerase activity (I, II, III, IV) were found. On the basis of ion exchanger affinity and poly(A), poly(C) and poly(Cm) replication ability, fraction I was classified as RNA-directed DNA polymerase of viral origin. On the basi...

journal_title：Acta biochimica Polonica

authors： Siedlecki JA,Mikke R,Zmudzka B

更新日期：1976-01-01 00:00:00

abstract：:Soluble alkaline phosphatase from Thiobacillus thioparus cells was purified about 230-fold. The enzyme had a mol. wt. of 50 000 daltons, optimum pH at 10.5, and was heat-resistant in the presence of diethanolamine. Polyacrylamide-gel electrophoresis demonstrated contamination of the preparation with inactive proteins ...

journal_title：Acta biochimica Polonica

authors： Galka M,Ostrowski W

更新日期：1976-01-01 00:00:00

abstract：:1. The everted-sac technique has been used to study the time-dependent effect of low-calcium diet on calcium active transport along rat small intestine. 2. In animals maintained on standard diet active translocation of calcium was limited to proximal 10 cm of the intestine. 3. In response to calcium restriction, calci...

journal_title：Acta biochimica Polonica

authors： Michalska L,Wróbel J,Szczepańska-Konkel M

更新日期：1976-01-01 00:00:00

abstract：:1. The activity of trypsin inhibitors in lung and pancreas increased with age of the animals. 2. In foetal lung the activity of the basic Kunitz-type inhibitor was 1% that, and in calf lung 5-30% that, found in adult animals. 3. In foetal pancreas the basic Kunitz-type inhibitor was not detected, and in calf pancreas ...

journal_title：Acta biochimica Polonica

authors： Wilusz T,Wilimowska-Pelc A,Mejbaum-Katzenellenbogen W

更新日期：1976-01-01 00:00:00